WebFeb 11, 2024 · 1.2.4流式细胞术检测细胞凋亡率 取各组PC12细胞加入预冷PBS洗涤,弃上清,加入结合缓冲液200 μl重悬细胞,分别加入5 μl膜联蛋白V异硫氰酸荧光素(Annexin V-FITC)与碘化丙啶(PI),避光孵育15 min,加入300 μl结合缓冲液后应用CytoFLEX流式细胞仪检测各组细胞凋亡率。 WebThe Flow Core supports a Beckman CytoFLEX S with 9 detectors and 4 lasers in CARE 2874 and a FACSCalibur 4 color flow cytometer system in CARE 2876. Access is available 24 hrs a day and 7 days per week once investigators are registered in Stratocore and approved by the core director. ... (PI, Sytox Orange, DsRed, TdTomato) 585/42 ECD …
CytoFLEX Trouble Shooting Guide - pedsresearch.org
WebBioLegend Your Partner for Antibodies, Proteins, Kits, Proteogenomics ... WebAdd 200 µl PI (from 50 µg/ml stock solution). Analysis of results. Measure the forward scatter (FS) and side scatter (SS) to identify single cells. Pulse processing is used to exclude cell doublets from the analysis. This can … raymond sillman flint michigan
A First Look at the Beckman Coulter CytoFLEX - CytoFluidix
WebSep 24, 2014 · A look inside the CytoFLEX revealing lots of unused space. The instrument itself is quite small, fitting roughly into a 40cm cube, but even the box itself seems to be too big for whats being housed inside. ... A pretty simple linearity test using PI stained CENs, and everything checks out as expected. However, like I mentioned earlier ... WebThe Cellaca PLX is the only multi-channel analysis instrument enabling rapid cell count, concentration, immunophenotyping, and viability readouts to significantly improve the … WebApr 6, 2024 · For apoptosis assay, the Annexin V-FITC/PI Apoptosis Detection Kit (Telenbiotech, China) was used. Briefly, cells were cultured with FBS-free culture medium. After 24 h starvation, cells were collected and stained with FITC and PI for 15 min at room temperature. The apoptotic cells were measured by flow cytometry (Cytoflex, Beckman). simplify 54/8