Optitype使用

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August undefined, 2024

WebJan 8, 2015 · OptiType is a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate 4-digit HLA genotyping predictions from … WebMay 20, 2024 · For both WES data and RNA-seq data, OptiType showed the highest accuracy for HLA-Ia typing than the other 3 programs at 2-digit and 4-digit resolution. Additionally, HLA typing accuracy from WES data was higher than from RNA-seq data (99.11% for WES data versus 96.42% for RNA-seq data). The accuracy of HLA-Ia typing by OptiType can …

工具篇 PHLAT：从转录组数据或者全外显子组测序数 …

WebMay 31, 2024 · Precision HLA typing from next-generation sequencing data - Issues · FRED-2/OptiType WebJul 19, 2024 · opitype利用HLA I型基因exon2到exon3的DNA序列构建了一个参考数据库，如果reads能够比对到这个参考数据库，认为这些reads来自与HLA基因。. 用法如下. docker … dickimouth

lohhla检测HLA区域LOH - 生物信息文件夹 - GitHub Pages

WebAug 20, 2014 · OptiType is applicable to NGS data of different sources and outperforms previously published in silico HLA typing approaches on both two- and four-digit resolution. The latter is especially important in clinical applications like individualized vaccine design, prevention of graft-versus-host disease and treatment of autoimmune diseases. ... WebJul 14, 2024 · OptiType, Phlat and Polysolver were used to execute HLA typing from WES data, while OptiType, Phlat and seq2hla were utilized for RNA-seq data. As shown in Figure 1, the accuracy of OptiType, Polysolver and Phlat for 4-digit HLA typing of WES data was 99.11%, 95.83% and 93.75%, respectively. Accuracy of OptiType, Phlat and seq2hla from … citizen white watch

OptiType on Biowulf - National Institutes of Health

WebDec 16, 2024 · conda install optitype 或者 docker pull fred2/optitype. 在使用OptiType进行HLA分析之前，建议先过滤出待分析序列中的HLA序列，这将能节省程序的运行时间，官方推荐使用RazerS3来进行比对，不过该软件会将序列读入到内存中，所以需要机器内存比较大。 WebInteractive jobs should be used for debugging, graphics, or applications that cannot be run as batch jobs. Allocate an interactive session and run the program. Sample session (user input in bold ): [user@biowulf]$ sinteractive --cpus-per-task 4 --gres lscratch:10 salloc.exe: Pending job allocation 46116226 salloc.exe: job 46116226 queued and ... dickie world of sportWeb与使用单个read相比，使用组装的contig可以大大减少给定序列信息的等位基因预测的模糊性。 HLA类型推断过程通常是通过各种计算方法来解决优化问题，例如贪婪算法（例如seq2HLA）、整数线性规划（例如 Optitype） … citizen white dial watch

"WebApr 14, 2024 · Optitype的运行需要python2（我实测使用python3也能成功运行），RazerS，samtools，HDF5，CPLEX（由于CPLEX是个商用软件，建议使用GPLK代 … " - Optitype使用

Optitype使用

lohhla检测HLA区域LOH - 生物信息文件夹 - GitHub Pages

WebJun 10, 2024 · OptiType is a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate 4-digit HLA genotyping predictions from NGS data by simultaneously selecting all major and minor HLA Class I alleles. Environment Modules. Run module spider optitype to find out what environment modules are available … WebJan 8, 2015 · Furthermore, depending on your settings in config.ini you can choose to keep the bam files OptiType produces when all-mapping reads against the reference: these will be stored in the output directory of your current run.. Then, if you want to re-run OptiType on the same sample, you can give it those intermediate .bam files as input instead of .fastq files, …

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WebNov 12, 2024 · nextNEOpi infers class-I and -II HLA types from WES/WGS (DNA-seq) and RNA-seq data using OptiType (Szolek et al., 2014) and HLA-HD (Kawaguchi et al., 2024), respectively, and can employ an RNA-seq-informed strategy to correct DNA-seq calls for missing HLA genes or alleles (Supplementary Methods).HLA typing benchmarking using … Web我们也做了一些原创性工作，在测试时发现OptiType有时会运行错误，便开发了一个补丁来解决这个问题，让OptiType能够稳定运行得到分型结果。另外，我们还基于打分策略开发了一个工具，整合肿瘤和对照样本的多个分型工具的分型结果，让分型准确性达到99.9% ...

WebJun 7, 2024 · For example, OptiType drops alleles that are not present in HLA databases, and HLAProfiler only selects the top n pairs by proportion of explained reads. Taking inspiration from HISAT-genotype, arcasHLA uses allele transcript quantification combined with culling of low-support alleles to narrow down the pool of possible alleles, thereby ... WebJun 25, 2024 · 使用全外显子组测序（wes）检测hla基因中的体细胞突变受到hla基因座的高度多态性的阻碍。描述一种计算管道，其能够准确推断I类HLA-A，-B和-C基因的种系等位 …

WebJun 10, 2024 · OptiType is a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate 4-digit HLA genotyping predictions from … WebOct 11, 2024 · Optitype. 软件安装. 最开始尝试使用docker，无奈悲剧的失败，发现bioconda有这个软件的，于是上conda，感觉比docker更方便呢。还有一个好处是，win10家庭版不支持docker，要想支持得修改注册表一通操作，太麻烦了。

WebJan 18, 2024 · I tried to install optitype as follow: conda install -c bioconda optitype I got the message: Collecting package metadata (current_repodata.json): done Solving environment: failed with initial frozen solve. Retrying with flexible solve. Solving environment: failed with repodata from current_repodata.json, will retry with next repodata source.

WebDec 1, 2014 · We present OptiType, a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate predictions from NGS data not … dickie wrightsil facebookWebOptiType is a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate 4-digit HLA genotyping predictions from NGS data by … dickie work shoes for menWebNov 22, 2024 · 使用Fastp对原始测序数据进行质量控制后，用OptiType、Phlat、Polysolver对WES数据执行HLA分型，用OptiType、Phlat和seq2hla对RNA-seq数据执行HLA分型，分别与基于SMRT技术的PCR-SBT方法获得HLA基因分型数据进行比较和准确性评估，从而对分型工具进行了性能评价，最终确定了 ... citizen windom mn newspaperWeblinux-64v1.3.2. noarchv1.3.5. conda install. To install this package run one of the following:conda install -c bioconda optitype. conda install -c "bioconda/label/cf202401" … dickieyo\u0027s frozen treatsWebJun 14, 2024 · optitype需要先利用razers3先过滤一下reads，把和HLA相关的序列提出来，然后在运行optitype进行分型，从而加快分析速度。第一步，Razers3 1 citizen windom mnWebJan 8, 2015 · OptiType is a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate 4-digit HLA genotyping predictions from … dickie wrights farnhamWebSep 10, 2024 · Optitype :只对ABC进行分型，据报道准确率较高，开发团队贴心地提供了在线版本。使用效果是对ABC的分型结果不错，目标区域测序数据分析耗时在1小时左右。幸 … dickie work shirts walmart