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Phospho foxo1

WebPhospho-FoxO1 (Ser256) UniProt ID: Q12778: NCBI: 2308: Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Description: FoxO1 (phospho-Ser256) antibody (FITC) Alternative names: WebPhospho-FoxO1 (Ser256) (E1F7T) recognizes endogenous levels of FoxO1 protein only when phosphorylated at Ser256. The antibody cross-reacts with overexpressed FoxO4 phosphorylated at Ser193 and may cross-react with overexpressed FoxO3a phosphorylated at Ser253. The antibody also cross-reacts with a protein of unknown origin around 160kD.

Anti-FOXO1A (phospho S256) antibody (ab131339) Abcam

WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). The Tunel assay … WebMar 27, 2024 · (A) Top: Representative immunoblots of phospho (p)-FOXO1/3a (Thr24/Thr32) and total FOXO1 from NRVM treated with BSA or myristate 500 μM (M500) for 24 h, unstimulated or stimulated with insulin 10 nM for 15 min. β-TUBULIN was used … list of iec 60079 standards https://ltdesign-craft.com

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WebPhospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody detects endogenous levels of Fox01/Fox03a only when phosphorylated at threonine 24 of … WebFOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the … WebThe Phospho-FoxO1 (Ser251) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize … imax theater in arkansas

FOXO1 ELISA Kits Biocompare

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Phospho foxo1

Anti-FOXO1A (phospho S256) antibody (ab131339) Abcam

WebThe ELISA kits listed below target FOXO1, the symbol for the human gene, forkhead box O1. The protein encoded by FOXO1 has a predicted amino acid length of 655, a mass of 69.7 kDa, and a cytoplasmic and nuclear subcellular localization. Pathologic involvement is reported in Rhabdomyosarcoma 2. WebFeb 25, 2011 · To determine which of the three FoxO1 phosphorylation sites at Thr 24, Ser 256, and Ser 319 are direct targets for PKA-α, we repeated the in vitro immune complex kinase assay represented in Fig. 1 with unlabeled ATP and examined the phosphorylation status of FoxO1 by immunoblotting anti-FLAG immunoprecipitates of transfected HEK293 …

Phospho foxo1

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WebSep 1, 2004 · The membranes were blocked with 5% nonfat dry milk, Tris-buffered saline, and 0.05% Tween 20 for 1 h at room temperature and then probed with anti-FoxO1 (Cell Signaling Technology, Beverly, MA) to detect FoxO1, anti-phospho-FoxO1 (Ser-256, Cell Signaling Technology) to detect phosphorylated FoxO1, and anti-α-tubulin (ICN) as an … WebNov 25, 2015 · # p values of <0.05 indicate significant differences from treatments without UP; (D) The levels of phospho-FOXO1 and phospho-p70S6K1 were measured after treatment with Akt inhibitor by Western blotting. Tubulin was detected as a loading control.

WebPhospho-FOXO1 (Ser256) This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar ... WebAug 2, 2005 · All reaction products were analyzed by Western blotting using anti-phospho-Foxo1 antibody or silver stain. Discussion. Our present study provides two findings about the consequences of Foxo1 acetylation, the attenuation of DNA-binding ability and the enhancement of the level of Foxo1 phosphorylation. We further explored the relevance of …

WebApr 13, 2024 · Treatment of cancer cells with PI3K inhibitors or taxane causes FOXO1 localization in the nucleus, increased expression of pERK1/2, and drug resistance. These effects are reversed by administering a small FOXO1-derived phospho-mimicking peptide inhibitor in vitro and in mice. Our results show a tumor suppressor role of AKT … WebPhospho-Fox01 (Ser256) Antibody detects endogenous levels of Fox01 only when phosphorylated at serine 256. The antibody cross-reacts with Fox04 phosphorylated at Ser193. Species Reactivity: Human, Mouse, Rat, Monkey Species predicted to react based …

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WebFOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the … list of ides for pythonimax theater hyderabadWebMar 27, 2024 · (A) Top: Representative immunoblots of phospho (p)-FOXO1/3a (Thr24/Thr32) and total FOXO1 from NRVM treated with BSA or myristate 500 μM (M500) for 24 h, unstimulated or stimulated with insulin 10 nM for 15 min. β-TUBULIN was used as a loading control. Bottom: Quantification of p-FOXO1/FOXO1 (n = 4). list of idioms to draw